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KMID : 0900919920160040311
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Korean journal of Animal Reproduction
1992 Volume.16 No. 4 p.311 ~ p.316
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Early Development Bovine Zygotes Co-cultured with Cumulus Cells
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Park Choon-Keun
Kim Choung-Ik
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Abstract
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Bovine follicular oocytes were matured, fertilized and cultured in vitro. Oocytes with or without cumulus cells were transferred into TC-I99 medium supplemented with 10% FCS 8 h after insemination, and embryos cleaved to 2- to 8-cell stage 56 h after insemination were cultured further for 5 days. The proportions of embryos developed to morular and blastocyst stages were significantly higher (P<0.0l) in those cultured with(31%) than without(15%) cumulus cells. When the embryos were cultured with cumulus cells in the medium with different protein sources, the highest proportion(59%) of embryos developed to morular or blastocyst stage was obtained in medium with BSA(P<0. O01). Higher proportions of embryos were degenerated during culture with FCS(15%) or CS(14%) compared with BSA(3%). The present results indicate the early development of in-vitro fertilized bovine embryos can be maintained efficiently by BSA when they were co-cultured with cumulus cells.
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